|
Stem Cell Transplantation; Expansion capacity of mesenchymal stem cells after sex-mismatched SCT is |
|
|
|
|
Tuesday, 08 November 2005 |
NewsRx.com
The expansion capacity of mesenchymal stem cells is damaged following sex-mismatched stem cell transplantation.
According to recent research from People's Republic of China, "The aim of this study was to investigate the expansion capacity and origin of bone marrow-derived mesenchymal stem cells (MSCs) in 34 patients who received a sex-mismatched stem cell transplant (SCT). Polymerase chain reaction (PCR) analysis of the amelogenin gene (AMEL) was used to detect donor-derived MSCs."
"Cultured MSCs were hybridized with fluorescence in situ hybridization (FISH) probes for chromosomes X and Y to distinguish cells of donor origin from those of host origin.
"The MSCs of 31 of the 34 patients showed confluent stroma, and the MSCs from 24 of these 31 patients were successfully passaged more than 5 times and were able to be used for PCR and FISH analyses," reported J. Wang and colleagues at Peking University in Beijing.
"The colony-forming unit-fibroblast, confluence time, and passage numbers of the MSCs and the colony-forming capacity of the hematopoietic progenitor cells of the patients were significantly different from those of 30 healthy control subjects," the authors said.
Wang continued, "Flow cytometry results showed that the proportion of CD14+CD45+ cells, which are regarded as monocytes/macrophages, in cultured MSCs (fifth passage) was less than 0.04%. PCR and FISH analyses revealed that the MSC-derived cells in all 24 patients were from the host."
"The expansion capacity of MSCs in patients who receive an SCT is damaged, and the MSCs originate from the host," investigators concluded.
Wang and colleagues published their study in International Journal of Hematology (Mesenchymal stem cells in stem cell transplant recipients are damaged and remain of host origin. Int J Hematol, 2005;82(2):152-158).
|
|
Last Updated ( Saturday, 24 December 2005 )
|
