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THE LONG TERM OBSERVATION OF OLFACTORY ENSHEATING GLIA TRANSPLANTATION TO RAT WITH ACUTE SPINAL CORD |
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Saturday, 24 December 2005 |
Jun Wu and Jian-Guo Shi
Department of Orthopaedics, Beijing Army General Hospital, Beijing, China
Objective: OEG transplatation is a promising strategy for spinal cord injury (SCI). In this study, we observe the long term results of OEG survival and repair in vivo for spinal cord contusion after the OEG transplantation.
Methods: The T13 spinal cord contusion of 2.5 months Wistar rat was made with the NYU impactor in Group A and B. OEG labeled by Hoechst was transplanted in group A. In group B, DMEM was injected. In Group C, T13 laminectomies were performed without SCI. The functional recovery was observed (BBB locomotion score) every 2 weeks. The retrograde labeling study was done postoperatively. HE and silver staining were also performed respectively to observe the pathological changes and axon regeneration. The survival of OEG labeled by Hoechst was observed by fluorescence microscope.
Results: 1. Locomotion behaviour had improved 6 weeks postoperatively. The BBB score of group A is higher than that of group B. The functional recovery appeared a platform at 16 weeks postoperatively (BBB score about 10). 2. Nerve fiber innervated injury area in the groups A and B. The number of nerve fibers of group A was more than that of group B (P<0.001), but less than that of group C (P<0.001). 3. A great number of OEG labeled by Hoechst were observed around spinal injury area. 4. There were abundant labeled neurons in the superior part of CNS including the intermediate zone and dorsal column of lower thoracic spinal cord, the upper thoracic, cervical spinal cord, reticular nucleus and raphe nucleus as well as reticular nucleus and parabrachial nucleus of pons.
Conclusion: 1. OEG can survive for at least 6 months in vivo, and can migrate from the injection sites. 2. For spinal cord contusion, OEG can promote the axons regeneration and a little recovery of locomotion function in our study.
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