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THE SURVIVAL, DIFFERENTIATION AND MIGRATION OF SKIN-DERIVED NEURAL STEM CELL IN INJURED SPINAL CORD |
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Saturday, 24 December 2005 |
Yuan-Shan Zeng1, Wei Zhang1 and Wu-Tian Wu2
1Department of Histology and Embryology, Sun Yat-sen University, Guangzhou, China
2Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong, China
Objective: We investigate whether the skin-derived neural stem cells inducted by the culture could survive, differentiate and migrate in the lesioned site of rat spinal cord.
Methodology: The skin of new born rat was applied to be dissociated into cells, cultured and inducted to proliferate in vitro. Skin-derived neural stem cells were identified. Then, skin-derived neural stem cells were transplanted into the lesioned site of rat spinal cord hemisection 30 days and 60 days after the operation.
Result: 10 days at culture, many cell-spheres had been formed by the proliferation of suspensive growing cells. Single cell separated once again from the cell-spheres could proliferate into a new cell-sphere in the culture. These cell-spheres showed nestin positive staining. 96 hours at the culture of containing serum, the anchoring neural stem cells could differentiate into MAP2, NF-200 and GFAP positive cells. At vivo, many transplanted cells were observed in the lesioned area of spinal cord. Some cells migrated into host spinal cord tissue. Transplanted cells showed nestin, MAP2, NF-200 and GFAP positive staining separately.
Conclusion: The results suggest that skin-derived neural stem cells inducted by the culture may survive, migrate and differentiate into neuron-like cells and astrocyte-like cells in injured spinal cord.
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