|
Bone Marrow Mononuclear Cell Transplantation into Heart Elevates the Expression of |
|
|
|
|
Tuesday, 03 August 2004 |
Yonggang Liu(a), Jingxuan Guo(a), Ping Zhang(a), Shaoheng Zhang(a), Ping Chen(b), Kangtao Ma(b), Chunyan Zhou(b)*
a: Department of Cardiology, the Third Hospital of Peking University
b: Department of Biochemistry and Molecular Biology, the School of Basic Medical Sciences, Peking University
Received 31 March 2004
Available online 3 August 2004
Abstract
The expression of angiogenic factors was measured to elucidate the mechanism of angiogenesis after bone marrow stem cells transplantation. The left anterior descending coronary (LAD) artery was ligated in male Lewis rats to induce an acute myocardial infarction (AMI); of which, some rats received bone marrow mononuclear cells transplantation (BMT). The expression of cytokines and their receptors was assessed by RT-PCR. The expression of c-met was upregulated in the AMI animals and was further increased when the animals received BMT ( P b 0.01). The other cytokines and their receptors remained unchanged after BMT compared with AMI samples. Normal bone marrow mononuclear cells have higher expression level of hepatocyte growth factor (HGF) than those in normal hearts. In conclusion, BMT could increase the expression of c-met. Not all angiogenic cytokines and their receptors were upregulated after BMT. The higher expression of hepatocyte growth factor in bone marrow may contribute to the upregulation of c-met.
Introduction
Ischemic heart disease has a high mortality and morbidity rate. When the coronary artery is thoroughly occluded, the heart may become necrotic. The inability of cardiac myocytes regeneration permanently affects heart functions when severely damaged, as is the case in the ischemic heart disease.
Bone marrow stem cells have been proven to have the ability of differentiating into cardiac myocytes (Jackson et al., 2001; Orlic et al., 2001; Toma et al., 2002; Tomita et al., 1999). Transplantation of bone marrow stem cells into the ischemic heart could lead to the regeneration of cardiac myocytes and to the recovery of the ischemic heart (Orlic et al., 2001; Perin et al., 2003; Strauer et al., 2001, 2002). In addition, cell transplantation promoted angiogenesis, which plays an important role in improving heart function (Fuchs et al., 2001). Evidence has shown that bone marrow stem cells could differentiate into endothelial cells and vascular smooth muscle cells; this would contribute to angiogenesis (Orlic et al., 2001). Bone marrow stem cells might also produce some angiogenic cytokines and upregulate endogenous cytokines expression to promote angiogenesis.
In this study, we tracked the expression of some angiogenic cytokines and their receptors to investigate which cytokines participated in the recovery of the infarct heart to normal function after bone marrow stem cells transplantation.
Conclusion
It is a complicated course that bone marrow cell engraftment improves cardiac function. Many cytokines, but not all, are involved in this course. The detailed molecular mechanisms remain to be further investigated.
|
|
Last Updated ( Tuesday, 15 November 2005 )
|
